| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Herr, Emily A (8)
|
MRTVSC |
42.78 |
42.14 |
-0.64 |
| 2 |
Panamserkhang, Tenzin P (8)
|
MRLGAC |
55.31 |
48.16 |
-7.15 |
| 3 |
Fried, Regan K (8)
|
MRLIAC |
51.40 |
50.67 |
-0.73 |
| 4 |
Pattanakul, Megan (7)
|
MRQNS |
53.00 |
51.33 |
-1.67 |
| 5 |
Frucchione, Emily A (8)
|
MRLIAC |
54.42 |
52.47 |
-1.95 |
| 6 |
Nassar, Noor (7)
|
MRLGAC |
54.06 |
52.61 |
-1.45 |
| 7 |
So, Leni A (7)
|
MRLIAC |
55.95 |
53.80 |
-2.15 |
| 8 |
Cahalane, Erin C (8)
|
MRTS |
54.58 |
53.86 |
-0.72 |
| 9 |
Lee, Cherry (8)
|
MRLIAC |
57.45 |
54.03 |
-3.42 |
| 10 |
Tan, Shanel E (7)
|
MRBBSC |
1:06.60 |
54.95 |
-11.65 |
| 11 |
Evenko, Veronica A (8)
|
MRFREE |
53.33 |
54.96 |
1.63 |
| 12 |
Klein, Farrah M (7)
|
MRTS |
1:12.94 |
56.35 |
-16.59 |
| 13 |
Gebhard, Kaitlyn A (8)
|
MRLIAC |
59.00 |
56.44 |
-2.56 |
| 14 |
Wilcox, Hailey (8)
|
MRHAA |
1:15.00 |
56.46 |
-18.54 |
| 15 |
Ulzheimer, Kiera E (8)
|
MRLIAC |
56.63 |
56.59 |
-0.04 |
| 16 |
Wu, Natalie (7)
|
MRLIAC |
58.39 |
57.49 |
-0.90 |
| 17 |
Karafin, Sasha (6)
|
MRLIAC |
58.83 |
57.67 |
-1.16 |
| 18 |
Lama, Tenzing L (7)
|
MRLGAC |
1:04.61 |
1:00.09 |
-4.52 |
| 19 |
Oberoi, Anya (8)
|
MRTS |
1:02.87 |
1:00.77 |
-2.10 |
| 20 |
Furnell, Amelia (8)
|
MRTS |
1:04.11 |
1:03.55 |
-0.56 |
| 21 |
Sforza, Sophia (8)
|
MRBBSC |
1:06.52 |
1:03.81 |
-2.71 |
| 22 |
Wilcox, Avery (6)
|
MRHAA |
1:25.00 |
1:04.01 |
-20.99 |
| 23 |
Kalinoglu, Samantha C (8)
|
MRLIAC |
1:08.33 |
1:04.61 |
-3.72 |
| 24 |
O'Sullivan, Brinan K (6)
|
MRLGAC |
1:18.94 |
1:06.03 |
-12.91 |
| 25 |
Secchi, Brooke D (8)
|
MRTS |
1:09.57 |
1:12.80 |
3.23 |
|
Ho, Rica L (8)
|
MRQNS |
58.60 |
DQ |
- |